To further study the pro-metastasis function of Gli1-derived S100A4 in pancreatic cancer cells, five groups of PC cells, L-Gli1i, L-C, L-Shh, L-Shh+siS100A4 MIS and L-Shh + siS100A4, were used to analize the regulation of Shh-Gli1 signals to S100A4, E-cadherin and VIM genes by real time RT-PCR and western-blotting assays. This evidence concerns the gene SHH and pachyonychia congenita.