Consistent with data obtained in immortalised glioma cell lines (Figures 3A and S2B), and with the fact that FAPi are capable of inhibiting FA pathway activation in primary glioma cell lines (Figures 2C, 2D and S1D); pre-treatment with curcumin, EF-24 or DDN all led to significantly increased TMZ sensitivity in several independently derived primary GBM cultures (Figure 3B and supplementary Figure S2C). This evidence concerns the gene FANCA and glioma.