Colon cancer HT-29 cells were treated with phloroglucinol (0, 12.5, 25 and 50 μg/ml), and then the percentages of necrotic and apoptotic cells were evaluated by staining with 7-aminoactinomycin D (7-ADD) and Annexin V. In each analysis, non-apoptotic viable cells showed negative staining with Annexin V and 7-AAD. Here, ANXA5 is linked to malignant colon neoplasm.