Although, steady state distribution of PTPD1 to actin filaments and adhesion plaques has been previously observed in primary fibroblasts [27], our data show that in cancer cells that over expressed PTPD1, EGFR/PTPD1-containing signalling complexes could be formed at membrane ruffles before EGF stimulation (Fig. 5b). This evidence concerns the gene EGFR and cancer.