All the patients with higher SCC-Ag level (SCC-Ag ≥2.0 ng/ml) before treatment had higher risks of advanced pathological tumor status (P<0.001), pathologic nodal metastasis lymph node ECS (P = 0.004), bone invasion (P<0.001), skin invasion (P<0.001) and tumor depth (>10 mm vs. ≤10 mm, P<0.001). This evidence concerns the gene RENBP and neoplasm.