This study was specifically undertaken to showcase the antioxidative potentials of L. fermentum Lf1, a promising indigenous probiotic Lactobacillus strain, to manage oxidative stress through “Nrf2” activation and modulation of lipid peroxidation by impacting MDA level under both in vitro (HT-29) and in vivo conditions in DSS colitis mouse model. The gene discussed is NFE2L2; the disease is colitis.