Earlier studies had already shown that Mirk depletion in each of four ovarian cancer cell lines grown adherent increased their toxic ROS levels by reducing expression of several antioxidant genes including superoxide dismutase 2 and ferroxidase (11), results consistent with the current studies in which spheroid cells made from two of these lines, OVCAR3 and SKOV3, were killed by Mirk kinase inhibition. This evidence concerns the gene SOD2 and ovarian carcinoma.