For comprehensive analysis of PTMs in PPARγ protein, we performed the affinity purification of PPARγ proteins from 293T cells and Caco-2 colon cancer cells, which were expressing exogenous FLAG-tagged PPARγ2 and endogenous PPARγ1 protein, respectively, using the scheme shown in Figure 1(a). Here, PPARG is linked to malignant colon neoplasm.