Because of their potential pathogenic role in CRMO, we performed the immunohistochemical staining of bone biopsy specimens from CRMO patients (n = 3), (Figure 2AI-FI) and from one tissue control (Figure 2 A-F) with antibodies to ASC, NLRP3, CASP-1 and IL-1β. The gene discussed is CASP1; the disease is chronic recurrent multifocal osteomyelitis.