We found that patients with NAFLD had significant differences in terms of BMI, SBP, DBP, ALT, AST, GGT, CK, TG, TC, HDL-C, LDL-C, FPG, UA, HbA1C, Hcy, Fe, SA, hsCRP, WBC, P-LIP, AMY, HGB, PLT and RDW compared with controls. This evidence concerns the gene CYGB and metabolic dysfunction-associated steatotic liver disease.