VTN and Parkinson disease: To investigate whether the interaction between DJ-1 and aSyn was influenced by PD-causing mutations in one of the partners, HEK293 cells were co-transfected with combinations of WT VN-Syn and familial mutant DJ-1-CFPC constructs or familial mutant constructs of VN-Syn with WT DJ-1-CFPC, respectively.