To further explore whether JNK/c-Jun signaling pathway is involved in HIF-1α protein accumulation induced by HPV-16 oncoproteins, two types of NSCLC cell lines were pretreated with SP600125, a specific JNK inhibitor, and HIF-1α protein expression was detected by Western blotting. The gene discussed is HIF1A; the disease is non-small cell lung carcinoma.