In addition, although autoantibodies against HSP70, ENO1 and RNH1 proteins were not significantly associated with histopathologic features, metastasis, or survival, plasma HSP70 and ENO1 autoantibodies levels were able to discriminate between carcinoma in situ or mucinous from invasive phenotype of CCA. Here, RNH1 is linked to in situ carcinoma.