ENO1 and in situ carcinoma: Remarkably, the comparison between the CCA invasive phenotype and carcinoma in situ or mucinous type, identified elevated HSP70 and ENO1 autoantibodies as significantly correlated with CCA invasiveness (7 cases had high OD vs 5 cases had low OD of autoantibodies for invasive type and 6 cases had low OD vs no cases with high OD of autoantibodies for carcinoma in situ or mucinous type, P = 0.038 by chi-square test).