EGF and neoplasm: HMT3522-T4-2_LBNL (T4-2) cells were generated from S1 cells by a multi-step process: 238 passages in medium without EGF followed by transplantation into a mouse which generated a tumor, and T4-2 cells were isolated from the serial passage of this tumor; thus T4-2 cells are malignant and tumorigenic [10].