As expected, administration of 100 μL of saline did not result in a decrement in hematocrit 7 days post‐AKI, suggesting that hemodilution and volume overload were not present; however, lung inflammation as judged by percent lung neutrophils, lung MPO activity, and lung CXCL1 accumulation were increased versus sham. This evidence concerns the gene CXCL1 and acute kidney injury.