TRERF1 and glioma: In this experimental model, RAPA and RAD significantly counteracted the increase in cell viability induced by the exposure to C-CM or LI-CM, although significantly higher concentrations (approximately 1,000-fold higher) were required to achieve a 50% reduction with respect to those found effective on C6 glioma cells (Figure 5B and C) or else on microglial cells not exposed to glioma factors [13].