In the present study, we investigated the Streptozotocin induced DM rat model at baseline 4 and 12 weeks post STZ injection to study: 1) the time dependent changes in urine biomarkers of NGF, PGE2; 2) differential expression of EP1and EP3 receptors, NGF and PGE2 in control and diabetes (hyposensitive bladder); 3) diabetes induced apoptotic changes. This evidence concerns the gene NGF and diabetes mellitus.