Although there were no differences in methylation or acetylation modification at histone H3 in CPCs and undifferentiated iPS cells derived from HLHS and BV patients, a marked decrease in dimethylated histone H3-lysine 4 (H3K4me2) and acetylated histone H3 (acH3) was found within the NKX2-5 promoter regions in differentiated HLHS-derived iPS cells compared with those from a BV patient. The gene discussed is NKX2-5; the disease is bacterial vaginosis.