HBA, HBD and HCD2 positively correlated with high-grade gliomas; GFAP, PHP14, 6PGL, PSD13, PEA15, TPIS, CRYAB_b, IPYR and IDH3A correlated with low-grade tumors; on the other hand, NFM, CN37, NDUS1 and MDHC negatively correlated with tumor samples. The gene discussed is SPAG1; the disease is central nervous system cancer.