In order to determine if TRPV1 mediates a portion of toxin A-induced colitis as it does ileitis, colonic segments were pretreated for 30 minutes with intraluminal administration of a TRPV1-defunctionalizing concentration of the TRPV1 partial agonist, I-RTX [22], before toxin A injection. Here, TRPV1 is linked to colitis.