Furthermore, even though TWEAK alone did not induce damage or apoptosis of intestinal epithelial cells, it was required, together with Fn14 and TNF, for IL-13-induced activation of caspase-3 in enterocytes isolated from γ-irradiated mice [39]. In vitro studies on rhabdomyosarcoma cell line Kym-1 showed that TWEAK upregulated expression of transmembrane TNF which in turn induced cell apoptosis via TNFR1 [183]. Here, TNFSF12 is linked to rhabdomyosarcoma.