We further demonstrated that N-ERC/mesothelin could be a useful biomarker for the early diagnosis of pleural mesothelioma and established an enzyme-linked immunosorbent assay (ELISA) using mAb clone 7E7 and clone 16K16 (7–16 ELISA) for the detection of N-ERC/mesothelin with relatively high sensitivity and specificity 19,20. This evidence concerns the gene MSLN and pleural mesothelioma.