When analyzing the distinct scores of expression of SFRP1 we found strong correlation of SFRP1 expression and the triple negative breast cancer subtype (Figure 1C).In addition, by using quantitative real time PCR and western blot analyses we found expression of SFRP1 in 5 out of 6 triple negative breast cancer cell lines (HCC-1937, MDA-MB-468, BT-20, MDA-MB-453 and HCC-1806), whereas no expression was detected in the TNBC cell line MDA-MB-231 as well as the non-TNBC cell lines SKBR-3 or MCF-7 (Figure 1D). The gene discussed is SFRP1; the disease is hepatocellular carcinoma.