Both promastigote- and amastigote-infected MΦs produced comparable levels of RNS at the time of infection (Figure S2A) and continued to produce similar concentrations of RNS up to 48 h post-infection (Figure S2B), suggesting that neither L. amazonensis promastigotes nor amastigotes interfere with iNOS production or activity in these primed cells. The gene discussed is NOS2; the disease is infection.