Using our previously described metagene analysis and S-score method (Rubin et al. 2011), we analyzed a global gene expression data set (Supplemental Tables 1, 2) of 62 PAX3:FOXO1+ and 24 PAX7:FOXO1+ human aRMS tumors for aberrant signaling of the rhabdomyosarcoma-associated p53 pathway. The gene discussed is FOXO1; the disease is rhabdomyosarcoma.