RT–PCR studies also showed higher levels of Pax3:Foxo1 mRNA levels in murine primary tumor cell cultures derived from Myf6Cre,Pax3(P3Fa/P3Fa) tumors (Fig. 2E, green) compared with primary tumor cell cultures from Pax7CreER,Pax3(P3Fa/P3Fa) tumors (U31425 and U28285) (Fig. 2E). This evidence concerns the gene FOXO1 and neoplasm.