FOXO1 and neoplasm: To determine whether the Pax locus could be pharmacologically modified, we first treated murine primary tumor cell cultures U23674 and U29415 with varying doses of 5-Aza 2′deoxycytidine (DNA methyltransferase inhibitor) or SAHA and entinostat (histone deacetylase [HDAC] inhibitors) to determine a sublethal dose at which cells could be treated to study the effects of these drugs on expression of Pax3:Foxo1 and Pax7 (Supplemental Fig. S5).