As additional validation we incorporated these hubs in a multivariate model which also included APE1, NBN, PTEN, PMS2 and MGMT. We found that FOXG1, THRA, RFX4, STXBP6, HPRT1, DCLK2 and TOGLN2 were independently prognostic along with APE1, NBN, PTEN, PMS2 and MGMT. Reassuringly, FOXG1, THRA, STXBP6 and RFX4 genes have previously been reported to be involved in glioma pathogenesis [41-43]. The gene discussed is PMS2; the disease is central nervous system cancer.