Transfection of this plasmid into the isogenic ccRCC cell lines revealed significantly enhanced SNAI1 promoter activity in the VHL-knockdown cells by 60% (SN12C-VHL) and 200% (ACHN-VHL) compared to the respective parental cell lines (Fig. 3B). The gene discussed is VHL; the disease is nonpapillary renal cell carcinoma.