In this study, IBS model rats had enhanced visceral pain sensitivity and reduced pain threshold; compared with the normal group, their PK2/PKR2 protein and mRNA expression in colon tissue were significantly increased (P < 0.05, P < 0.01), indicating that PK2 and PKR2 are associated with enhanced visceral pain sensitivity in IBS model rats. Here, PROKR2 is linked to irritable bowel syndrome.