TWIST1 and intrahepatic cholangiocarcinoma: In the present study we compared a multimarker (MM) RT-qPCR assay, consisting of keratin 19 (KRT19), TWIST1, and mammaglobin A (hMAM), with ICC using the AE1-AE3 mAb for the detection of DTCs in 267 early breast cancer patients previously treated with adjuvant fluorouracil, cyclophosphamide, epirubicin (FEC) chemotherapy.