Western blot analysis of endogenous Uev1s using a Uev1-specific monoclonal antibody LN2B [39] could only detect Uev1C in several breast cancer cell lines including MDA-MB-231 and MCF7, and MCF10A, an immortalized normal mammary epithelial cell line (Figure 1B). Here, UBE2V1 is linked to breast carcinoma.