Since all mice in the study were introduced into a novel tethered chamber for the recording period, and heightened anxiety behavior has been documented in Mecp2 null mice (Lang et al., 2014); the first wake cycle durations (i.e., latency to first sleep cycle) were analyzed and found to be longer in KO mice compared to WT (Figure 1C5), however not significantly (p = 0.06). This evidence concerns the gene MECP2 and Anxiety.