After pancreatic cancer Panc-1 cells expressing a high level of OCT4 were stably transfected with Lv-shOCT4, the mRNA and protein expression levels of OCT4 and AKT were detected by qPCR (Fig. 3A and B) and western blot analysis (Fig. 3C–F). This evidence concerns the gene AKT1 and familial pancreatic carcinoma.