After pancreatic cancer Panc-1 cells expressing a high level of OCT4 were stably transfected with Lv-shOCT4, the mRNA and protein expression levels of OCT4 and AKT were detected by qPCR (Fig. 3A and B) and western blot analysis (Fig. 3C–F). The gene discussed is POU5F1; the disease is familial pancreatic carcinoma.