POU5F1 and familial pancreatic carcinoma: The expression of OCT4 was detected in pancreatic cancer cells with different degrees of differentiation (Bxpc3, Panc-1 and Mia PaCa-2) by qPCR (Fig. 2A) and western blot analysis (Fig. 2B and C), of which OCT4 was highly expressed in the Panc-1 cell line compared with the other ones (P<0.01).