For example, forced expression of Bcl-2 protein from plasmid vectors, in contrast, abrogates sensitivity to the apoptosis promoting effects of antiestrogens in breast cancer lines, while antisense Bcl-2 prevents estrogen-mediated apoptosis suppression, thus establishing a direct functional connection between ER and Bcl-2 and suppression of apoptosis [35]. The gene discussed is ESR1; the disease is breast carcinoma.