MAPK8 and acute liver failure: We and other researchers previously reported that NAS effectively inhibited the release of Cyt-C and AIF from the mitochondria into the cytoplasm in apoptotic cells under oxidative stress injury induced by H2O2 and melatonin [35], while melatonin protected AIF-dependent cell death in a model of acute liver failure through its direct inhibition of hepatic RIP1 and subsequent JNK phosphorylation [53].