The present study revealed that PI3K and p-Akt are detected in advanced NSCLC, however, no significant difference was identified between the staining location and overexpression of PI3K and p-Akt in stage IIIB–IV NSCLC tissues compared with that in stage I–IIIA NSCLC tissues. This evidence concerns the gene AKT1 and non-small cell lung carcinoma.