Antibody specificity was confirmed in several breast cancer cell lines (T47D, ZR-75-1 and SKBR3), whereby PRDX1 expression was modified using short hairpin loop RNA (shRNA)-mediated gene knockdown and overexpression of cDNA encoding V5-tagged PRDX1, before antibody validation by immunoblotting (Figure 1A and C; complete gel displayed in Figure S1A in Additional file 3), RPPA analysis (Figure 1B) and IHC (Figure 1D and E). The gene discussed is PRDX1; the disease is breast cancer.