The present study was designed to determine the effects of choline on modulating hepatic TG accumulation, DNA methylation of PPARα, mRNA expression of the critical genes and their enzyme activities involved in hepatic lipid metabolism, ROS levels, Δψm and GSH-Px activity through in vitro models of cellular steatosis using a combination of excessive energy substrates (lactate, pyruvate and octanoate, LOP). Here, PPARA is linked to steatosis.