Eight significantly mutated genes were identified, among of which six genes are well-known tumor-associated genes (TP53, RB1, CDKN2A, PIK3CA, NOTCH1, and NFE2L2), whereas two have not previously been described in ESCC (ADAM29 and FAM135B).21 The gene discussed is FAM135B; the disease is esophageal squamous cell carcinoma.