Accumulation of LC3 punctas in osteosarcoma cells was significantly higher in the Dox (0.2 μg/ml) or Cis (20 μM) treatment groups (Fig. 1A); there were more GFP-positive dots (autophagic vesicles) in the Dox- or Cis-treated Saos-2 cells when compared with the control Saos-2 cells (P<0.05; Fig. 1B). Here, MAP1LC3A is linked to osteosarcoma.