LRRK2 and Parkinson disease: We compared the basal phosphorylation in cells to the in vitro autophosphorylation of PD mutated LRRK2 using anti-GFP immunoprecipitates from HEK293 T-REx cells expressing GFP-LRRK2 WT, Arg1441Cys, Arg1441Gly, Ala1442Pro, Tyr1699Cys, Ile2012Thr, Gly2019Ser, and Ile2020Thr in a tetracycline inducible manner, Figure 2A.