The previous suggestion that SOX2OT plays a role in regulating chromatin structure [36] has been extended, to demonstrate a specific effect of SOX2OT on SOX2 regulation, using both expression arrays from human breast cancers, a series of breast cancer cell lines and a single line exhibiting ectopic expression of SOX2OT. The results add to the growing picture that emphasizes the importance of non-coding RNA in tumor cell properties. The gene discussed is SOX2-OT; the disease is breast cancer.