Because Smad3 was recently shown to interact with Notch1 in esophageal adenocarcinoma cells after treatment with TGF-β, we tested if N1ICD binding to the Sox9 promoter RBP-Jκ site was dependent on TGF-β signaling in lung ADC. The gene discussed is TGFB1; the disease is esophageal adenocarcinoma.