We mutated the four putative RBP-Jκ binding sites, as well as one additional site (Site 1) proposed by Song et al. [37] to cooperate with the Smad3 SBE (Smad binding element) upstream of Sox9 in esophageal adenocarcinoma (Fig. 4B). Here, SMAD3 is linked to esophageal adenocarcinoma.