To further investigate the in vivo effect of rVP1, instead of pretreating CL1-5GL cells with rVP1, we implanted the cancer cells into SCID mice and one week later start administering rVP1 (5 or 15 mg/kg body weight) intravenously via the tail vein three times per week for 4 weeks. This evidence concerns the gene CLDN3 and cancer.