To investigate whether rVP1-mediated decrease of COX-2/PGE2 (and/or MIG-7) is functionally associated with rVP1/integrin β1/Akt-mediated reduction in EMT, MMP2 and invasion of lung cancer cells, we transfected lung cancer cell lines (H1299 and CL1-5) with COX-2 or MIG-7 plasmids (pCOX2 or pMIG7) to overexpress COX-2 or MIG-7 protein. This evidence concerns the gene PTGS2 and lung cancer.