PTGS2 and lung carcinoma: Constitutive phosphorylation of Akt at serine 473 (AktS473) by transfecting lung cancer cells with dominant active Akt plasmid (pAkt-DA) increased the EMT, MMP2 activity and invasion ability of cancer cells and increased expression of COX-2 and MIG-7 (Figure 2B and 2C).