Thus, although the possibility that EE might impact other systemic effects of diabetes, such as insulin resistance, HbA1c, blood pressure, and stress hormone expression cannot be formally ruled out, the present results suggest that EE behaved as an anti-VEGF, anti-inflammatory, anti-oxidative therapy, and increased BDNF in the context of retinal damage induced by early diabetes. This evidence concerns the gene BDNF and Insulin resistance.