Knockdown of aldh7a1 by Aldh7a1 MO1in zebrafish embryos resulted in bent tail (Figure S3A, D in File S1); coloboma of the eye evident at 28 hpf which persists until at least 5–6 days post-fertilization (dpf) (Figure 2B,D; Figure S3 E,F in File S1) compared to control embryos (Figure 2A,C; Figure S3B,C in File S1) where the optic fissure had nearly fused at 28 hpf (Figure 2A). The gene discussed is ALDH7A1; the disease is coloboma.