AKT1 and hepatocellular carcinoma: To evaluate whether PI3K/AKT signals could mediate the invasive properties of PRL-1 in HCC, stably transfected Huh7-HA-PRL-1 cells were treated with PI3K inhibitor, ZSTK474 (10 uM) which abrogated the enhanced migration mediated by PRL-1 in HCC cells as measured by the wound-healing assay (Figure 4E) and the matrigel invasion assay (p<0.01, Figure 4F).