To take account of potential off-target effects of AMPK activating drugs, we used two structurally unrelated, commonly used AMPK activators, 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) and metformin [42, 43], and compared their transcriptional effects in prostate cancer cells. This evidence concerns the gene PRKAA1 and Familial prostate cancer.