PRKAA1 and Familial prostate cancer: To investigate whether AMPK-regulated genes might have a role in prostate cancer, we used gene set enrichment analysis (GSEA) [48, 49] to compare our set of AMPK target genes to three published expression datasets comparing benign/normal prostate tissue with primary and metastatic prostate cancer (Grasso, Taylor and Varambally datasets) [50-52].