While targeting Bit1 expression in the mitochondria inhibits anoikis resistance and anchorage-independent growth of NSCLC cells, specific downregulation of endogenous Bit1 in these cells further potentiated their anoikis insensitivity and anchorage-independent growth in vitro and tumorigenic growth in vivo. Here, PTRH2 is linked to non-small cell lung carcinoma.