In the present study, we constructed lentiviral vectors to knock down NLRP3 to evaluate the role of the NLRP3 inflammasome in atherosclerosis in apolipoprotein (Apo) E-deficient mice, which can develop severe hypercholesterolemia and spontaneous atherosclerosis [12]. The gene discussed is NLRP3; the disease is Hypercholesterolemia.