Considering that Gankyrin exerts its oncogenic function by regulating the phosphorylation of Rb and the ubiquitylation of p535, 8, 9, 10, 11, 12, we examined by IHC the expression of p53 and Rb as well as Gankyrin in 193 HCC samples with available follow-up data. This evidence concerns the gene RB1 and hepatocellular carcinoma.